Coding
yA
Part:BBa_K678035:Design
Designed by: DTU-Denmark-2 Group: iGEM11_DTU-Denmark-2 (2011-09-04)
yA (green pigment) from Aspergillus nidulans
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1176
Illegal XbaI site found at 380
Illegal PstI site found at 1540
Illegal PstI site found at 1927 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1176
Illegal NheI site found at 1742
Illegal PstI site found at 1540
Illegal PstI site found at 1927 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1176
Illegal BamHI site found at 1453
Illegal BamHI site found at 1736
Illegal XhoI site found at 87 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1176
Illegal XbaI site found at 380
Illegal PstI site found at 1540
Illegal PstI site found at 1927 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1176
Illegal XbaI site found at 380
Illegal PstI site found at 1540
Illegal PstI site found at 1927
Illegal NgoMIV site found at 1577 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 54
Illegal BsaI site found at 1616
Design Notes
This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains the tails at the 5' and 3' ends as shown on the figure below.
Source
Aspergillus nidulans genomic DNA (AN6635)
References
(1) O’Hara, E.B., and Timberlake, W.E. (1989) Molecular Characterization of the Aspergillus nidulans yA Locus. Genetics 121: 249-254.